A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR

Ching Yu Chou, Minglee Chu, Chi Fon Chang, Tai-huang Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Analysis of NMR relaxation data has provided significant insight on molecular dynamic, leading to a more comprehensive understanding of macromolecular functions. However, traditional methodology allows relaxation measurements performed only at a few fixed high fields, thus severely restricting their potential for extracting more complete dynamic information. Here we report the design and performance of a compact high-speed servo-mechanical shuttle assembly adapted to a commercial 600 MHz high-field superconducting magnet. The assembly is capable of shuttling the sample in a regular NMR tube from the center of the magnet to the top (fringe field ∼0.01 T) in 100 ms with no loss of sensitivity other than that due to intrinsic relaxation. The shuttle device can be installed by a single experienced user in 30 min. Excellent 2D- 15N-HSQC spectra of (u- 13C, 15N)-ubiquitin with relaxation at low fields (3.77 T) and detection at 14.1 T were obtained to illustrate its utility in R 1 measurements of macromolecules at low fields. Field-dependent 13C-R 1 data of (3,3,3-d)-alanine at various field strengths were determined and analyzed to assess CSA and 1H- 13C dipolar contributions to the carboxyl 13C-R 1.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalJournal of Magnetic Resonance
Volume214
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Magnets
Molecular Dynamics Simulation
Ubiquitin
Alanine
Nuclear magnetic resonance
high speed
Equipment and Supplies
nuclear magnetic resonance
Superconducting magnets
high resolution
Macromolecules
Molecular dynamics
assembly
high field magnets
alanine
superconducting magnets
macromolecules
field strength
magnets
methodology

Keywords

  • Field cycling
  • Field-dependent NMR relaxation
  • High-speed sample shuttler
  • Molecular dynamics
  • Nuclear magnetic relaxation dispersion (NMRD)
  • Servo-mechanical shuttler

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this

A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR. / Chou, Ching Yu; Chu, Minglee; Chang, Chi Fon; Huang, Tai-huang.

In: Journal of Magnetic Resonance, Vol. 214, 01.01.2012, p. 302-308.

Research output: Contribution to journalArticle

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