Purpose: The purpose of this study was to investigate the acute effect of whole-body vibration exercise with or without weight-bearing on the intermittent cycling sprint performance. Methods: Twelve college male athletes (age, 20.6 ± 1.0 yrs; height, 176.3 ± 5.3 cm; weight, 68.7 ± 5.3 kg) voluntarily participated in this repeated measures designed and counter balanced study. All subjects were asked to respectively perform three treatments, including vibration (Whole-Body Vibration, WBV, 30 Hz, ± 0.8 mm), vibration with weight-bearing (Vibration With Weight-Bearing, WWBV, 30 Hz, ± 0.8 mm, 40% body weight), and nonvibration (Control, CON, ± 0 Hz, 0 mm) exercise treatment before the 6 × 10-sec Intermittent Cycling Sprint (ICS) test. During the treatments, each subject was asked to perform the 10 × 30-sec treatment with 1-min resting interval in a halfsquat position on the vibration platform. After the treatments, subjects performed the ICS test, used to assess the peak and mean power, fatigue index and time to peak power, with 1-min resting interval on a cycling ergometer. The capillary blood samples were obtained from the fingertips at the baseline, immediately after treatments, the middle of the ICS test, and 5-min after the ICS test to measure the blood lactate concentration. Results: There were no significant differences on the time to peak power and fatigue index of the ICS test among the three treatments (p ＞ .05). The mean powers of the 5th sprint in the WBV and WWBV were significantly higher than that in the CON (WBV vs. WWBV vs. CON, 571.50 ± 105.08 vs. 571.10 ± 102.91 vs. 544.88 ± 93.28W, p ＜ .05). The peak power of the 6th sprint in the WBV was significantly higher than that in the CON (WBV vs. CON, 715.83 ± 134.50 vs. 636.83 ± 102.77W, p ＜ .05). The blood lactate levels at immediately after the treatment in the WWBV were significantly higher than those in the WBV and CON (WBV vs. WWBV vs. CON, 1.55 ± 0.79 vs. 2.60 ± 1.27 vs. 1.40 ± 0.61 mmol/L, p ＜ .05). Conclusions: This study shows that acute WBV with or without additional load might improve the performance in the later stage during an intermittent cycling sprint test, and the performance improvement after acute WBV might be better than that after vibration with additional load.