Purpose: This study aimed to investigate the effects of different amplitudes whole-body vibration (WBV) on the explosive performance, hormone and surface electromyography (EMG) activities. Methods: Twenty male college athletes voluntarily participated in this study. All subjects were assigned to four treatments, included general warm up (WUP), non-vibration exercise (CON, 0 Hz, 0 mm), low-amplitude (LAT, 30 Hz, 1.6 mm,1.45 g), and high-amplitude (HAT, 30 Hz, 2.8 mm, 2.54 g) treatments by randomized, balanced order designed. Each subject performed 1min × 10 sets (1min rest interval) WBV on the vibration platform with half-squat position. Explosive performance tests consisting of counter movement jump (CMJ) and 30 seconds continue jump (CJ). EMG and blood samples were recorded from the treatments. The data were analyzed by repeated one-way ANOVA and LSD post hoc test. Results: There were no significant differences on the CMJ and CJ performances, lactate, testosterone and cortisol levels. In the CMJ test, the EMGrms of biceps femoris in LAT were significantly lower than those in HAT, WUP and CON (p＜.05). In the CJ test, the median frequency of right biceps femoris in LAT was significantly higher than those in HAT and CON (p＜.05), and the median frequency of left biceps femoris was also significantly higher than those in the other treatments (p＜.05). Conclusion: These results indicated that the different amplitudes (≤2.8 mm) of vibration exercise could not improve the power performance; however, the low-amplitude of WBV might delay the fatigue of the biceps femoris during power endurance exercise.