The purpose of this study was to investigate the physiological effects of the whole-body vibration (WBV) exercise with different low frequencies during the recovery phase after high-intensity intermittent sprint exercise. Eighteen male college students were recruited to complete this randomized crossover experiment. After high-intensity intermittent cycling sprints (HIIS) (6 x 10-s), all subjects were asked to respectively perform three treatments, no vibration (15 min, 0 Hz, ± 0 mm) and 6 Hz (15 min, ± 3 mm) and 12 Hz (15 min, ± 3 mm) vibration exercises, on the vibration platform. Immediately after the treatment, each subject was requested to perform the 10-s high-intensity cycling sprint (10-s HIS) to clarify the acute recovery effects of WBV. And 24 hours after the treatment, subjects needed to perform the HIIS test again to confirm the chronic recovery effects of WBV. The power output profiles, heart rates, blood lactate concentrations and rates of perceived exertion (RPE) were measured during the HIIS test and the 10-s HIS test. There were no significant differences on the peak power, mean power and total work at each sprint of HIIS test and 10-s HIS test among the three treatments (p＞.05). The blood lactate concentrations and RPE during HIIS test and 10-s HIS test were also showed no significant differences among the three treatments (p＞.05). The results indicated that the WBV with low frequencies (6 or 12 Hz) could not improve the recovery of cycling sprint performance and the elimination of fatigue after intense intermittent sprints.