As the nucelic acid amplification tests has been widely used by clinicians, polymerase chain reaction (PCR) technique plays an important role. This method can amplify a DNA sequence via the modulating temperature control, and the number of nucleic acid amplification can achieve diagnosis. The purpose of this study is to develop a portable, fast, and small-volume on-chip sensing devices for nucleic acids amplification tests. Herein the ultrafast laser process and Nanowires process techniques were used to design and fabricate microheater, and to design and fabricate the thin-film-based the modulating temperature-control devices. An on-chip device of this study can perform exponential DNA amplification in vitro through precise thermal control at each step of the polymerase chain reaction (PCR). In the design of PCR devices, the type of ultra-fast laser is used the picosecond laser to directly fabricate the electrode structure with the surface properties of hole arrays on a multilayer graphene (MLG). The mechanism of surface device structures can achieve stable and uniform thermal control, and integrate the program control system for the amplification of BK virus DNA fragments. The results of the study showed that the design of the patterned hole-array structure devices can be formed and achieved the amplification and diagnosis of the BK virus in the 354-bp DNA fragment of the marker VP1. Study demonstrates further the feasibility of thin-film electrode with MLG as the heater and in vitro PCR detection and evaluation in a programmable PCR system device. It will be helpful to develop new platform technology of nucleic acid amplification, which can have the opportunity to the practical biomedical detection.
|Effective start/end date||2017/08/01 → 2018/10/31|
- Thermal stability and uniformity
- Nucelic acid amplification
- Thin-film devices
- Ultrafast laser process
- Picosecond laser ablation
- Multilayer graphene
- Nanowire process
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