Deep-sea water (DSW) from 200 meters below sea level is non-polluted cool and clean water full of a variety of ions and organic species with high bioactivity. In the ocean there are many known and undiscovered substances, many of which become valuable to human through concentration, filtration and purification. DSW occupied 95% sea water in the earth. Therefore, DSW as cycle materials can be continuously and richly supplied from the sea water. Among DSW, the higher value of biomedical application of the bioactive molecules is importantly worth exploring and acquiring. Our preliminary data found that an extract of 1070 molecules from DSW efficiently inhibited the growth of Helicobacter pylori in vivo and in vitro. This novel finding rejected the thinking that high [Ca2+] and [Mg2+] affect the bacterial growth and biomedical effects. In addition, this novel finding is the newest discovery and has been applied with several patents in the world. We further determine the biomedical effects of these possible bioactive molecules on the Helicobacter pylori growth, Atopic dermatitis, lipid profiles and atherosclerosis in the animal models. How to purify, characterize, mass produce and valuably develop of these biomedical molecules from DSW to biomedical application is the urgent target for high-valued cycle materials establishment. This four-year project will be performed by the team of National Taiwan Normal University, Stone and Resource Industry R&D Center and National Taiwan University Hospital to extract, characterize, assay and apply to biomedical exploration. Therefore, this project aims to study the characteristics of DSW and tries to understand its physical and chemical properties. Using micro liquid-liquid extraction technique and fiber-spray mass spectrometry to identify and to analyze the bioactive species obtained from DSW. Other spectroscopic studies will also be incorporated to establish the pattern characteristic of its physiological activity. In addition, we will isolate and purify the organic ion possessing bioactivity, and further to identify the structure of the bioactive species by NMR and crystallography. We will explore the biomedical function of these high-valued bioactive molecules from cycle materials DSW in the animal model and clinical human trial. Finally, the pilot production and mass production of the high-valued cycle materials of biomolecules from DSW will be prepared by Stone and Resource Industry R&D Center and industry, respectively. We have finished the protocol, characteristics identification and biomedical application of the DSW bioactive species about 40% of the entire project. We have got data to the degree of 100% of expected results. We now have partly processed the isolation, chemical formula and structures of the DSW bioactive species for the patent and new drugs. Our preliminary data also displayed that the isolated DSW bioactive species exert the inhibition on the growth of Helicobacter pylori and ameliorate gastroesophageal reflux diseases in the rat models.
|Effective start/end date||2020/06/01 → 2021/05/31|
- deep-sea water (DSW)
- bioactive molecules
- high-valued biomedical application
- crystal growth
- structural determination
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