氧化石墨烯量子點於電漿子特性及microRNAs感測機制之基礎研究

This project is the 1 years phase in Fundamental studies of graphene oxide quantum dots for plasmonic properties and microRNAs in sensing mechanism. The sera contain stable cell-free miRNAs, small non-coding RNAs with important regulatory roles in proliferation, apoptosis and cell-cell communication, which can be identified and quantified, making them the ideal biomarker candidates for non-invasive diagnostic/prognostic purposes. Moreover, aberrant microRNAs (miRNAs) expression has been recently linked to pregnancy complications, such as preeclampsia (PE). Thus, we aimed to assess the potential of circulating miRNAs as sensitive and specific biomarkers for the early diagnosis of preeclampsia. The Graphene Oxide Quantum Dot (GOQDs) based SPR a prominent assay designed to detect and quantify mature miRNAs in a fast, specific, accurate and reliable manner. This work demonstrates the excellent potential of functionalized GOQDs (GOQD–COOH and GOQD–AuNPs) composites to form biocompatible surfaces on sensing films for use in fluorescent and SPR-based miRNAs affinity biosensors. Functionalization of GOQDs can modulate its visible spectrum, and can therefore be used to improve and control the plasmonic coupling mechanism. The functionalized GOQDs (f-GOQDs) based SPR biosensors have three advantages; including high performance, high sensitivity and excellent molecular kinetic response. As a powerful platform, miRNAs techniques could also be used for identification of new biomarkers for PE screening. In the current study, we have evaluated the potential of miRNA-210 as a novel high detection rates biomarker for screening pregnancies of PE in clinical serum samples. In summary, the excellent binding affinity, low detection limit, high sensitivity, good stability and specificity suggest the potential of this GOQDs-based SPR chip detection method in clinical application. The development of real-time serum sample analytic and early diagnostic tools to detect abnormalities at an early stage of pregnancy is a promising technique for future clinical application of specific microRNAs.