官能化二維層狀過渡金屬材料於奈米電漿子特性及生物分子感測機制之基礎研究

This project is the 1 years phase in Fundamental studies of 2DLMs functionalized transition metal dichalcogenides (TMDs) for nano-plasmonic properties with advanced biosensing mechanisms. The results show that the fMoS2 layers can be served as effective light absorption medium. Under resonance conditions, the electrons in these additional dielectric layers can be transferred to the surface of gold thin film. The results showed that the fMoS2 layers could compensate the SPR effect of gold and significantly improve the sensitivity of the biosensor. The development of real-time whole blood analytic and diagnostic tools to detect abnormalities at an early stage of pregnancy is a promising technique for future clinical applications. In this study, functionalized MoS2 chips (f-MoS2 chip) were prepared in two ways. The carboxyl acid groups (-COOH) of oxalic and chloroacetic acid (MCA) were modified to the surface of MoS2 to carry out the quality of the sensing material comparison. Which MCA modification technology, mainly chlorine atoms occupy sulfur vacancies, have a high stability and binding efficiency, different from the traditional method of thiol ligand modifications, in this instance, they yield physisorbed disulfides that are easily removed. The results of XPS analysis showed the content of carboxyl group was 24.4% in C1s and 28.2% in O1s, and f-MoS2 was confirmed by XPS, FTIR, TEM, TEM, UPS and Zeta potential. In the biological detection, low injection (20 μL) and low flow rate (6 μL / min) in bovine serum albumin (BSA) immunoaffinity detection were carried out to compare with traditional SPR chip sensitivity in the presence of f-MoS2 (MCA) chip sensitivity increased by 2.35 times. In the PAPP-A2 recombinant protein experiment, the R2=0.99, and after adding the interference does not affect the sensitivity and linearity. PAPP-A2 in clinical serum samples, the sample was diluted to 40000 times, and the SPR angle of PAPP-A2 at a concentration of about 0.05 pg/mL could still be detected, R2 = 0.958. We demonstrated that f-MoS2 chip has high sensitivity in the detection of clinical samples. PAPP-A2 has a high specificity and has the potential to become a new indicator protein for future diagnosis of pregnancy disorders, which is expected to be a screening protein for Down's syndrome and epilepsy, achieving rapid screening and high detection rates in the future.