Whole rock O-isotopes of the Panjal Traps are presented in order to assess the influence of crustal contamination and hydrothermal alteration in their genesis. The basalts from the eastern Kashmir Valley and Lidder Valley have enriched δ18OVSMOW values (9.0‰ to 12.0‰). The basalt with the lowest δ18OVSMOW values (9.0‰ and 9.2‰) were likely affected by deuteric alteration but their values could be close to the original melt composition as the rocks do not show trace element (Th/NbPM ≈0.8; Nb/U ≈ 50; Th/La ≈0.1) or isotopic evidence of crustal contamination (87Sr/86Sri = 0.7043 to 0.7045; εNd(t) = +1.1 to +1.3). The δ18OVSMOW (> 12‰) values and Nd isotopes (εNd(t) < −8.6) of the silicic Panjal Traps are consistent with derivation from continental crust. The remaining mafic rocks have enriched Sr-Nd-O isotopic values that indicate crustal (10–30%) contamination (εNd(t) = −1.9 to −6.1; 87Sr/86Sri = 0.7051 to 0.7087; δ18O = 10.1‰ to 12.0‰) with the upper flows exhibiting further enrichment by hydrothermal alteration. The basalts from the Pir Panjal Range, western Kashmir Valley, have variable Nd isotopic values (εNd(t) = −6.8 to +4.3) and the lowest δ18OVSMOW values (6.8‰ to 7.9‰) of the study. The results demonstrate that the rocks from the Pir Panjal Range preserved not only differences in radiogenic isotopes but also the O-isotopes as well. The change in the Nd and O isotopes of the basalt from Guryal Ravine and Lidder Valley to the more depleted values of the Pir Panjal Range is likely due to a transition from a chondritic mantle source to a depleted mantle source as the continental rift evolved to a sea-floor spreading environment.